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45-430 staining (1ug/ml) of Human Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
45-430 staining (1ug/ml) of Human Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

CREM Antibody

CATALOG NUMBER: 45-430

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: LETLKDICSPKTDY
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 35kDa band observed in Human Heart lysates (calculated MW of 32.5kDa according to NP_853549.1). Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is expected to recognise the following protein isoforms of human CREM protein: a (NP_853549.1); e (NP_874387.1); f (NP_874388.1); k (NP_874393.1); l (NP_874394.1); m (NP_877570.1); n (NP_877571.1); o (NP_877572.1); p (NP_877573.1); q (NP_878270.1); v (NP_898883.1). This antibody is not expected to cross-react with CREB protein.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CREM
Additional Names:
CREM, ICER, MGC17881, MGC41893, MGC111110, hCREM-2, cAMP responsive element modulator, hCREM 2alpha-b protein, hCREM 2beta-a protein, inducible cAMP early repressor ICER, OTTHUMP00000019442, OTTHUMP00000019443, cAMP response element modulator, hCREM 2alpha-b protein,
Protein Accession Number:
NP_853549.1,NP_874387.1,NP_874388.1,NP_874393.1,NP_874394.1,NP_877570.1,NP_877571.1,NP_877572.1,NP_877573.1,NP_878270.1,NP_898883.1
Protein Gi Number:
34335186
Ncbi Gene Id Number:
1390
Background Reference 1:
Tenbrock K, Juang YT, Tolnay M, Tsokos GC. The cyclic adenosine 5'-monophosphate response element modulator suppresses IL-2 production in stimulated T cells by a chromatin-dependent mechanism. J Immunol. 2003 Mar 15;170(6):2971-6.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-430

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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