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Western Blot at 1:1000 dilution + PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of CREM Antibody in Hela cell line lysates (35ug/lane)
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.

CREM Antibody

CATALOG NUMBER: 63-790

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This CREM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-314 amino acids from the C-terminal region of human CREM.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CREM
Additional Names:
cAMP-responsive element modulator, Inducible cAMP early repressor, ICER, CREM
Protein Accession Number:
Q03060
Protein Gi Number:
259016392
Ncbi Gene Id Number:
1390
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CREM is a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes.
Background Reference 1:
Fujimoto,T., et.al., J. Biochem. 115 (2), 298-303 (1994)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-790

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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