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Western blot analysis of CRH in human placenta tissue lysate with CRH antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of CRH in human brain tissue with CRH antibody at 5 μg/mL.
Immunofluorescence of CRH in human brain tissue with CRH antibody at 20 μg/mL.
Western blot analysis of CRH in human placenta tissue lysate with CRH antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

CRH Antibody

CATALOG NUMBER: 7797

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
CRH antibody was raised against a 16 amino acid peptide near the amino terminus of human CRH.
The immunogen is located within amino acids 30 - 80 of CRH.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
CRH antibody can be used for detection of CRH by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
CRH antibody is human specific.
Positive Control 1:
Cat. No. 1309 - Human Placenta Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 22 kDa
Observed: 29 kDa
Purification:
CRH antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CRH antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CRH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
CRH
Additional Names:
CRH Antibody: CRF, Corticoliberin, Corticotropin-releasing factor, CRF
Protein Accession Number:
NP_000747
Protein Gi Number:
4503041
Ncbi Gene Id Number:
1392
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Corticotropin-releasing hormone (CRH) is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress (1). In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta where it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturitio (2). Marked reduction in this protein has been observed in association with Alzheimer disease (3).
Background Reference 1:
Maras PM and Baram TZ. Sculpting the hippocampus from within: stress, spines, and CRH. Trends Neurosci. 2012; 315-24.
Background Reference 2:
Vrachnis N, Malamas FM, Sifakis S, et al. Immune aspects and myometrial actions of progesterone and CRH in labor. Clin. Dev. Immunol. 2012; 937618.
Background Reference 3:
Leake A, Perry EK, Perry RH, et al. Neocortical concentrations of neuropeptides in senile dementia of the Alzheimer and Lewy body type: comparison with Parkinson's disease and severity correlations. Biol. Psychiatry 1991; 29:357-64.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7797

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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