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Western blot analysis of extracts of various cell lines, using CRHBP antibody (22-319) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat heart using CRHBP antibody (22-319) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using CRHBP antibody (22-319) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using CRHBP antibody (22-319) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CRHBP antibody (22-319). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CRHBP antibody (22-319) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

CRHBP Antibody

CATALOG NUMBER: 22-319

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-322 of human CRHBP (NP_001873.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
Rat brain
Positive Control 2:
Rat kidney
Predicted Molecular Weight:
Observed: 36kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CRHBP
Additional Names:
CRF-BP, CRFBP, corticotropin-releasing factor-binding protein, CRF-binding protein, CRH-BP
Ncbi Gene Id Number:
1393
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Corticotropin-releasing hormone is a potent stimulator of synthesis and secretion of preopiomelanocortin-derived peptides. Although CRH concentrations in the human peripheral circulation are normally low, they increase throughout pregnancy and fall rapidly after parturition. Maternal plasma CRH probably originates from the placenta. Human plasma contains a CRH-binding protein which inactivates CRH and which may prevent inappropriate pituitary-adrenal stimulation in pregnancy.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-319

List Size:
100 uL

List Price:

$436.00

Datasheet
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