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Western Blot at 1:8000 dilution Lane 1: T47D whole cell lysate Lane 2: THP-1 whole cell lysate Lysates/proteins at 20 ug per lane.
CRIP1 Antibody IHC analysis in formalin fixed and paraffin embedded bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:8000 dilution Lane 1: T47D whole cell lysate Lane 2: THP-1 whole cell lysate Lysates/proteins at 20 ug per lane.

CRIP1 Antibody

CATALOG NUMBER: 61-633

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This CRIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-77 amino acids from the C-terminal region of human CRIP1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:8000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
9 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CRIP1
Additional Names:
Cysteine-rich protein 1, CRP-1, Cysteine-rich heart protein, CRHP, hCRHP, Cysteine-rich intestinal protein, CRIP, CRIP1, CRIP, CRP1
Protein Accession Number:
P50238
Protein Gi Number:
20981688
Ncbi Gene Id Number:
1396
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cysteine-rich intestinal protein (CRIP) belongs to the LIM/double zinc finger protein family, members of which include cysteine- and glycine-rich protein-1 (CSRP1; MIM 123876), rhombotin-1 (RBTN1; MIM 186921), rhombotin-2 (RBTN2; MIM 180385), and rhombotin-3 (RBTN3; MIM 180386). CRIP may be involved in intestinal zinc transport.
Background Reference 1:
Garcia-Barcelo, M., et al. Genomics 47(3):419-422(1998)
Background Reference 2:
Khoo, C., et al. Protein Expr. Purif. 9(3):379-387(1997)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-633

List Size:
400 ul

List Price:

$495.00

Datasheet
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