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42-282 (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-282 (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

CRIPTO Antibody

CATALOG NUMBER: 42-282

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: YGRNCEHDVRKEN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Immunohistochemistry:In paraffin embedded Human Placenta shows staining assocated with nuclei in some trophoblasts and weak cytoplasm staining in cytotrophoblasts. Recommended concentration, 3-5ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TDGF1
Additional Names:
TDGF1, teratocarcinoma-derived growth factor 1, CR, CRGF, CRIPTO, Cripto-1, -
Protein Accession Number:
NP_003203.1
Protein Gi Number:
4507425
Ncbi Gene Id Number:
6997
Background Reference 1:
Parisi S, D1Andrea D, Lago CT, Adamson ED, Persico MG, Minchiotti G Nodal-dependent Cripto signaling promotes cardiomyogenesis and redirects the neural fate of embryonic stem cells J Cell Biol. 2003 Oct 27;163(2):303-14

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-282

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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