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Western blot analysis of lysate from MDA-MB-231 cell line, using CRISP1 Antibody at 1:1000 at each lane.
Western blot analysis of lysate from MDA-MB-231 cell line, using CRISP1 Antibody at 1:1000 at each lane.

CRISP1 Antibody

CATALOG NUMBER: 59-344

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CRISP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162-191 amino acids from the C-terminal region of human CRISP1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CRISP1
Additional Names:
Cysteine-rich secretory protein 1, CRISP-1, AEG-like protein, ARP, Acidic epididymal glycoprotein homolog, CRISP1, AEGL1
Protein Accession Number:
P54107
Protein Gi Number:
1706134
Ncbi Gene Id Number:
167
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Fertilization consists of a sequence of specific cell-cellinteractions culminating in the fusion of the sperm and egg plasmamembranes. Recognition, binding, and fusion occur through theinteraction of complementary molecules that are localized tospecific domains of the sperm and egg plasma membranes. In thesperm, the postacrosomal region or equatorial segment is involvedin sperm-egg plasma membrane fusion. The protein encoded by thisgene is a member of the cysteine-rich secretory protein (CRISP)family. This protein is expressed in the epididymis, is secretedinto the epididymal lumen, and binds to the postacrosomal region ofthe sperm head where it plays a role at fertilization in sperm-eggfusion through complementary sites localized on the egg surface.Two isoforms are encoded by transcript variants of this gene.
Background Reference 1:
Mungall, A.J., et al. Nature 425(6960):805-811(2003)
Background Reference 2:
Evans, J.P. Hum. Reprod. Update 8(4):297-311(2002)
Background Reference 3:
Cuasnicu, P.S., et al. Arch. Med. Res. 32(6):614-618(2001)
Background Reference 4:
Cohen, D.J., et al. Biol. Reprod. 65(4):1000-1005(2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-344

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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