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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with 42-521 (0.11ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 42-521 (1mg/ml) in L
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with 42-521 (0.11ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 42-521 (1mg/ml) in L

CRISP2 Antibody

CATALOG NUMBER: 42-521

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Pig, Dog, Cow
Immunogen:
The immunogen for this antibody is: KCTLQHSDPEDRK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing Human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.05-0.1ug/ml.
Specificity:
Reported variants represent identical protein: NP_003287.1, NP_001135879.1, NP_001135889.1, NP_001135907.1, NP_001135880.1.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CRISP2
Additional Names:
CRISP2, cysteine-rich secretory protein 2, CRISP-2, GAPDL5, MGC111136, TPX1, TSP1, OTTHUMP00000016589, glyceraldehyde-3-phosphate dehydrogenase-like 5, testis specific protein 1 (probe H4-1 p3-1)
Protein Accession Number:
NP_003287.1
Protein Gi Number:
4507671
Ncbi Gene Id Number:
7180

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-521

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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