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Western blot analysis of CRISP2 in human testis tissue lysate with CRISP2 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of CRISP2 in human testis tissue with CRISP2 antibody at 10 μg/mL.
Immunofluorescence of CRISP in human testis tissue with CRISP antibody at 20 μg/mL.
Western blot analysis of CRISP2 in human testis tissue lysate with CRISP2 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

CRISP2 Antibody

CATALOG NUMBER: 5873

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
CRISP2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human CRISP2.
The immunogen is located within the first 50 amino acids of CRISP2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
CRISP2 antibody can be used for detection of CRISP2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2:
Cat. No. 11-701 - Human Testis Tissue Slide
Purification:
CRISP2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CRISP2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CRISP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CRISP2
Additional Names:
CRISP2 Antibody: CT36, TPX1, TSP1, GAPDL5, CRISP-2, Cysteine-rich secretory protein 2, Testis-specific protein TPX-1
Protein Accession Number:
NP_001135880
Protein Gi Number:
215422434
Ncbi Gene Id Number:
7180
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CRISP2 Antibody: The cysteine-rich secretory proteins (CRISP) family is a group of four proteins that are strongly expressed in the male reproductive tract and have been implicated in having roles in male fertility. CRISP2, also known as TPX1, has been implicated in the adhesion between spermatids and Sertoli cells, and with CRISP1, is thought to be involved in sperm-egg fusion. CRISP2 has been shown to regulate the Ca2+ influx through ryanodine receptors (RYR) and may influence the acrosome reaction or sperm motility. CRISP2 has also been shown to bind to the mitogen-activated protein kinase kinase kinase 11 (MAP3K11) and localizes to the developing acrosome, suggesting this CRISP2-MAP3K11 complex may have a role in acrosome development.
Background Reference 1:
Cohen DJ, Busso D, Da Ros V, et al. Participation of cysteine-rich secretory proteins (CRISP) in mammalian sperm-egg interaction. Int. J. Dev. Biol.2008; 52:737-42.
Background Reference 2:
Maeda T, Sakashita M, Ohba Y, et al. Molecular cloning of the rat Tpx-1 responsible for the interaction between spermatogenic and Sertoli cells. Biochem. Biophys. Res. Commun.1998; 248:140-6.
Background Reference 3:
Busso D, Goldweic NM, Hayashi M, et al. Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusion. Biol. Reprod.2007; 76:701-8.
Background Reference 4:
Gibbs GM, Scanlon MJ, Swarbrick J, et al. The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signaling. J. Biol. Chem.2006; 281:4156-63.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5873

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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