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45-041 (0.01ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-041 (3.8ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-041 (0.01ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

CRKL Antibody

CATALOG NUMBER: 45-041

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-KIFDPQNPDENE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in lysates of cell lines K562 (calculated MW of 33.8kDa according to NP_005198.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Thymus shows staining of cellular membranes in thymocytes. Recommended concentration: 2.5-3.8ug/ml.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CRKL
Additional Names:
CRKL, v-crk sarcoma virus CT10 oncogene homolog (avian)-like, v-crk avian sarcoma virus CT10 oncogene homolog-like, OTTHUMP00000198539
Protein Accession Number:
NP_005198.1
Protein Gi Number:
4885153
Ncbi Gene Id Number:
1399
Background Reference 1:
Singer CF, Hudelist G, Lamm W, Mueller R, Handl C, Kubista E, Czerwenka K. Active (p)CrkL is overexpressed in human malignancies: potential role as asurrogate parameter for therapeutic tyrosine kinase inhibition. Oncol Rep. 2006 Feb;15(2):353-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-041

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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