Western blot analysis of various lysates using CRM1/XPO1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

Western blot analysis of lysates from mouse lung, using CRM1/XPO1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3min.

Immunofluorescence analysis of C6 cells using CRM1/XPO1 Rabbit mAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using CRM1/XPO1 Rabbit mAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using CRM1/XPO1 Rabbit mAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of various lysates using CRM1/XPO1 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.

CRM1/XPO1 Rabbit Recombinant Antibody (81-538)

CATALOG NUMBER: 81-538

Clonality:

Monoclonal

Tested Applications:

ELISA, IF/ICC, WB

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Immunogen:

Synthetic peptide. This information is considered to be commercially sensitive.

Conjugate:

Unconjugated

Tested Applications:

ELISA, IF/ICC, WB

Application Note:

WB,1:1000 - 1:2000, IF/ICC,1:100 - 1:400, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Positive Control 1:

U2OS,MCF7,HeLa,Mouse thymus,Mouse lung

Predicted Molecular Weight:

123kDa

Advanced Validation

Properties

purification:

Affinity purification

Clonality:

Monoclonal

Isotype:

IgG

Physical State:

Liquid

Buffer:

Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Concentration:

Batch dependent

Storage Conditions:

Store at -20℃. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

XPO1

Additional Names:

emb; CRM1; exp1; CRM-1; Exportin 1; CRM1/XPO1

Protein Accession Number:

O14980

Ncbi Gene Id Number:

7514

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

This cell-cycle-regulated gene encodes a protein that mediates leucine-rich nuclear export signal (NES)-dependent protein transport. The protein specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. This protein also regulates NFAT and AP-1.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

This product is for research use only.

CATALOG NUMBER:

81-538

List Size:

100 uL

List Price:

$477.00

Datasheet

Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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Bulk Pricing Available

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Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

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CRM1/XPO1 Rabbit Recombinant Antibody (81-538)

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Positive Control 1:

Predicted Molecular Weight:

purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Ncbi Gene Id Number:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

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