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Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Antibody used in WB on Human HepG2 at 2.5 ug/ml.

CROT Antibody

CATALOG NUMBER: 30-463

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CROT.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
CROT antibody can be used for detection of CROT by ELISA at 1:312500. CROT antibody can be used for detection of CROT by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
70 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store CROT antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
CROT
Additional Names:
CROT, COT
Protein Accession Number:
NP_066974
Protein Gi Number:
31542325
Ncbi Gene Id Number:
54677
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Carnitine octanoyltransferase is a carnitine acyltransferase that catalyzes the reversible transfer of fatty acyl groups between CoA and carnitine. This provides a crucial step in the transport of medium- and long-chain acyl-CoA out of the mammalian peroxisome to the cytosol and mitochondria.Carnitine octanoyltransferase (EC 2.3.1.137) is a carnitine acyltransferase that catalyzes the reversible transfer of fatty acyl groups between CoA and carnitine. This provides a crucial step in the transport of medium- and long-chain acyl-CoA out of the mammalian peroxisome to the cytosol and mitochondria. See also CRAT (MIM 600184). Van der Leij et al. (2000) [PubMed 11001805] reviewed the function, structural features, and phylogenetics of human carnitine acyltransferase genes, including CROT.
Background Reference 1:
Morillas, M., (2002) J. Biol. Chem. 277 (13), 11473-11480.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-463

List Size:
100 ul

List Price:

$473.00

Datasheet
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