Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain tissue reacted with CROT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

CROT Antibody

CATALOG NUMBER: 63-919

Clonality:

Polyclonal

Tested Applications:

Flow, IHC-P, WB

Host Species:

Rabbit

Species Reactivity:

Human, Mouse

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse

Immunogen:

This CROT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-79 amino acids from the N-terminal region of human CROT.

Conjugate:

Unconjugated

Tested Applications:

Flow, IHC-P, WB

Application Note:

For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:50~100
For WB starting dilution is: 1:1000

Predicted Molecular Weight:

70 kDa

Advanced Validation

Properties

Purification:

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Clonality:

Polyclonal

Isotype:

Rabbit Ig

Physical State:

Liquid

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Storage Conditions:

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

Ncbi Official Symbol:

CROT

Additional Names:

Peroxisomal carnitine O-octanoyltransferase, COT, CROT, COT

Protein Accession Number:

Q9UKG9

Protein Gi Number:

48429265

Ncbi Gene Id Number:

54677

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

CROT encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.

Background Reference 1:

Li, Y.L., et al. FEBS J. 276(1):303-314(2009)

Background Reference 2:

Morillas, M., et al. J. Biol. Chem. 277(13):11473-11480(2002)

Background Reference 3:

van der Leij, F.R., et al. Mol. Genet. Metab. 71 (1-2), 139-153 (2000) :

Background Reference 4:

Ferdinandusse, S., et al. Biochem. Biophys. Res. Commun. 263(1):213-218(1999)

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-919

List Size:

400 ul

List Price:

$495.00

Datasheet

Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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CROT Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Protein Gi Number:

Ncbi Gene Id Number:

User Note:

Background:

Background Reference 1:

Background Reference 2:

Background Reference 3:

Background Reference 4:

Disclaimer:

List Size:

Shipping Info

Customize your order

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