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45-433 (0.2ug/ml) staining of Human (A), Mouse (B) and Rat (C) Kidney lysates (35ug protein in RIPA buffer). Detected by chemiluminescence
HEK293 overexpressing CRP2 and probed with 45-433 (mock transfection in first lane).
45-433 (3.8ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-433 (0.2ug/ml) staining of Human (A), Mouse (B) and Rat (C) Kidney lysates (35ug protein in RIPA buffer). Detected by chemiluminescence

CRP2 Antibody

CATALOG NUMBER: 45-433

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Horse
Immunogen:
The immunogen for this antibody is: C-ERLGIKPESVQP
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 26kDa band observed in Human, Mouse and Rat Kidney lysates (calculated MW of 21.0kDa according to NP_001312.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Huber et al, J Biol Chem. 2000 Feb 25;275(8):5504-11; PMID: 10681529). In transfected HEK293 transiently expressing CRP2 a band of approx. 26kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Lung shows staining of the endothelial cells of a blood vessel. Recommended concentration: 3-6ug/ml.
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CSRP2
Additional Names:
CRP2, CSRP2, cysteine and glycine-rich protein 2, LMO5, SmLIM, LIM domain only 5, smooth muscle
Protein Accession Number:
NP_001312.1
Protein Gi Number:
4503101
Ncbi Gene Id Number:
1466
Background Reference 1:
Stearns ME, Wang M, Hu Y, Garcia FU. Interleukin-10 activation of the interleukin-10E1 pathway and tissue inhibitor of metalloproteinase-1 expression is enhanced by proteasome inhibitors in primary prostate tumor lines. Mol Cancer Res. 2003 Jul;1(9):631-42. Erratum in: Mol Cancer Res. 2003

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-433

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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