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45-436 (2ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
45-436 (2ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Cryopyrin Antibody

CATALOG NUMBER: 45-436

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-DLYEKAKRDEPK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 70kDa band in human lymph node and tonsil lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 118kDa according to NP_004886.3 and 106kDa according to NP_899632.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Tonsil shows focal and interstitial staining of the inflammatory elements close to the epithelia of the tonsil crypts. Recommended concentration: 2-4ug/ml.
Specificity:
This antibody is expected to recognise all five reported isoforms (NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1). Reported variants represent identical protein (NP_004886.3 and NP_001073289.1).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NLRP3
Additional Names:
CIAS1, cold autoinflammatory syndrome 1, AGTAVPRL, AII/AVP, C1orf7, FCAS, FCU, MWS, NALP3, PYPAF1 , AII/AVP receptor-like, Muckle-Wells syndrome, NACHT, LRR and PYD containing protein 3, PYRIN-containing APAF1-like protein 1, angiotensin/vasopressin receptor AII/AVP-like, cryopyrin
Protein Accession Number:
NP_004886.3,NP_899632.1,NP_001230062.1,NP_001120933.1,NP_001120934.1
Protein Gi Number:
34878693
Ncbi Gene Id Number:
114548
Background Reference 1:
Kanneganti TD, Ozoren N, Body-Malapel M, Amer A, Park JH, Franchi L, Whitfield J, Barchet W, Colonna M, Vandenabeele P, Bertin J, Coyle A, Grant EP, Akira S, Nunez G. Bacterial RNA and small antiviral compounds activate caspase-1 through cryopyrin/Nalp3. Nature. 2006 Mar 9;440(7081):233-6. Epub 2006 Jan 11.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-436

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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