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Western blot analysis of lysate from U-937 cell line, using CSF1R Antibody at 1:1000.
Confocal immunofluorescent analysis of CSF1R Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysate from U-937 cell line, using CSF1R Antibody at 1:1000.

CSF1R Antibody

CATALOG NUMBER: 63-033

Clonality:
Polyclonal
Tested Applications:
Flow, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CSF1R antibody is generated from rabbits immunized with CSF1R recombinant protein.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
108 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CSF1R
Additional Names:
Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms, CD115, CSF1R, FMS
Protein Accession Number:
P07333
Protein Gi Number:
547770
Ncbi Gene Id Number:
1436
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CSF1R is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases.
Background Reference 1:
Guey,L.T., et.al., Eur. Urol. 57 (2), 283-292 (2010)
Background Reference 2:
Wooten,E.C., et.al., PLoS ONE(1), E8830 (2010)
Background Reference 3:
Hosgood,H.D. III, et.al., Respir Med 103 (12), 1866-1870 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-033

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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