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Western blot analysis in HL60 cell line lysates (35ug/lane).
CSF2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of CSF2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis in HL60 cell line lysates (35ug/lane).

CSF2 Antibody

CATALOG NUMBER: 55-344

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CSF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-85 amino acids from the Central region of human CSF2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
16 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CSF2
Additional Names:
Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, CSF, Molgramostin, Sargramostim, CSF2, GMCSF
Protein Accession Number:
P04141
Protein Gi Number:
117561
Ncbi Gene Id Number:
1437
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CSF2 is a cytokine thatcontrols the production, differentiation, and function ofgranulocytes and macrophages. The active form of the protein isfound extracellularly as a homodimer. This gene has been localizedto a cluster of related genes at chromosome region 5q31, which isknown to be associated with interstitial deletions in the 5q-syndrome and acute myelogenous leukemia. Other genes in the clusterinclude those encoding interleukins 4, 5, and 13. [provided byRefSeq].
Background Reference 1:
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Background Reference 2:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 3:
Beckers, M.M., et al. Eur. J. Intern. Med. 21(4):289-292(2010)
Background Reference 4:
Wang, Y., et al. Diabet. Med. 27(4):376-383(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-344

List Size:
400 ul

List Price:

$495.00

Datasheet
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