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Western Blot at 1:1000 dilution Lane 1: Caco2 whole cell lysate Lane 2: KG-1 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with CSF3R Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution Lane 1: Caco2 whole cell lysate Lane 2: KG-1 whole cell lysate Lysates/proteins at 20 ug per lane.

CSF3R Antibody

CATALOG NUMBER: 63-768

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CSF3R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 252-280 amino acids from the Central region of human CSF3R.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
92 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CSF3R
Additional Names:
Granulocyte colony-stimulating factor receptor, G-CSF receptor, G-CSF-R, CD114, CSF3R, GCSFR
Protein Accession Number:
Q99062
Protein Gi Number:
729564
Ncbi Gene Id Number:
1441
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CSF3R is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variants encoding four different isoforms have been found for this gene, with three of the isoforms being membrane-bound and the other being secreted and soluble.
Background Reference 1:
Yerges,L.M., et.al., J. Bone Miner. Res. 24 (12), 2039-2049 (2009)
Background Reference 2:
Germeshausen,M., et.al., Blood 114 (16), 3504-3505 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-768

List Size:
400 ul

List Price:

$495.00

Datasheet
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