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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CK1a antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-CK1a Pab in A375 cell lysate
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CK1a antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

CSNK1A1 Antibody

CATALOG NUMBER: 62-883

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Xenopus
Immunogen:
This CSNK1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-337 amino acids from the C-terminal region of human CSNK1A1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CSNK1A1
Additional Names:
Casein kinase I isoform alpha, CKI-alpha, CK1, CSNK1A1
Protein Accession Number:
P48729
Protein Gi Number:
31077177
Ncbi Gene Id Number:
1452
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CK1a can phosphorylate a large number of proteins. This cytoplasmic protein participates in Wnt signaling. It has been demonstrated to phosphorylate CTNNB1 on Ser-45 and to interact with the Axin complex.
Background Reference 1:
Liu, C., et al., Cell 108(6):837-847 (2002).
Background Reference 2:
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Background Reference 3:
Fish, K.J., et al., J. Biol. Chem. 270(25):14875-14883 (1995).
Background Reference 4:
Tapia, C., et al., FEBS Lett. 349(2):307-312 (1994).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-883

List Size:
400 ul

List Price:

$495.00

Datasheet
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