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Western blot analysis of anti-CK1d C-term Pab in HeLa cell lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of anti-CK1d C-term Pab in HeLa cell lysate

CSNK1D Antibody

CATALOG NUMBER: 62-884

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This CSNK1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 321-352 amino acids from the C-terminal region of human CSNK1D.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CSNK1D
Additional Names:
Casein kinase I isoform delta, CKI-delta, CKId, Tau-protein kinase CSNK1D, CSNK1D, HCKID
Protein Accession Number:
P48730
Protein Gi Number:
27923980
Ncbi Gene Id Number:
1453
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The casein kinase 1 (CK1) group consists of 12 kinases including CK1, TTBK (tau tubulin kinase), and VRK (vaccinia-related kinase) families.The receptor guanylate cyclase (RGC) group consists of 5 kinases similar in domain sequence to TKs (ANP, CYG).
Background Reference 1:
Sillibourne, J.E., et al., J. Mol. Biol. 322(4):785-797 (2002).
Background Reference 2:
Rivers, A., et al., J. Biol. Chem. 273(26):15980-15984 (1998).
Background Reference 3:
Kusuda, J., et al., Genomics 32(1):140-143 (1996).
Background Reference 4:
Fish, K.J., et al., J. Biol. Chem. 270(25):14875-14883 (1995).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-884

List Size:
400 ul

List Price:

$495.00

Datasheet
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