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Western blot analysis of extracts of various cell lines, using CSNK1E antibody (18-170) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts from normal (control) and CSNK1E knockout (KO) HeLa cells, using CSNK1E antibody (18-170) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat pancreas using CSNK1E Antibody (18-170) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CSNK1E Antibody (18-170) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using CSNK1E antibody (18-170). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CSNK1E antibody (18-170) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
KO

CSNK1E Antibody, KO Validated

CATALOG NUMBER: 18-170

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 247-416 of human CSNK1E (NP_689407.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:100
Positive Control 1:
HeLa
Positive Control 2:
293T
Predicted Molecular Weight:
Observed: 47kDa
Validation:
Antibody is Knockout validated.
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CSNK1E
Additional Names:
CSNK1E, casein kinase 1, epsilon, RP1-5O6.1, HCKIE, MGC10398, CKI-epsilon, OTTHUMP00000063258, OTTHUMP00000063263, casein kinase 1 epsilon, casein kinase I epsilon, CKIepsilon
Ncbi Gene Id Number:
1454
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-170

List Size:
100 uL

List Price:

$540.00

Datasheet
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