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43-693 (1ug/ml) staining of NIH3T3 (A), HeLa (B), K562 (C), MCF7 (D) and HepG2 (E) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-693 (0.3ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Testis lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-693 (1ug/ml) staining of NIH3T3 (A), HeLa (B), K562 (C), MCF7 (D) and HepG2 (E) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CSNK2B Antibody

CATALOG NUMBER: 43-693

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-TGLNEQVPHYRQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in lysates of cell lines NIH3T3, HeLa, K562, MCF7 and HepG2 (calculated MW of 24.9kDa according to NP_001311.3). Approx 25kDa band observed in Mouse Testis, 26kDa in Rat Testis and 28kDa in Pig Testis lysates (calculated MW of 24.9kDa according to Mouse NP_001290374.1, Rat NP_001030315.1 and pig NP_001138849.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001311.3; NP_001269314.1).
Predicted Molecular Weight:
Approx 26kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CSNK2B
Additional Names:
CSNK2B, casein kinase 2, beta polypeptide, CK2B, CK2N, CSK2B, G5A, CK II beta, Casein kinase II beta subunit, alternative name: G5a, phosvitin, casein kinase II subunit beta, phosvitin, protein G5a
Protein Accession Number:
NP_001311.3,NP_001269314.1
Protein Gi Number:
23503295
Ncbi Gene Id Number:
1460
Background Reference 1:
French AC, Luscher B, Litchfield DW.Development of a stabilized form of the regulatory CK2beta subunit that inhibits cell proliferation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-693

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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