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Western blot analysis of extracts of various cell lines, using CSRP1 Antibody (13-771) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug CSRP1 antibody (13-771). Western blot was performed from the immunoprecipitate using CSRP1 antibody (13-771) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using CSRP1 Antibody (13-771) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.

CSRP1 Antibody

CATALOG NUMBER: 13-771

Clonality:
Polyclonal
Tested Applications:
IP, RIP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human CSRP1 (NP_004069.1).
Conjugate:
Unconjugated
Tested Applications:
IP, RIP, WB
Application Note:
WB: 1:500 - 1:2000
IP: 1:50 - 1:200
RIP: 1:20 - 1:50
Positive Control 1:
A-549
Positive Control 2:
BT-474
Positive Control 3:
HeLa
Positive Control 4:
Mouse lung
Positive Control 5:
Mouse brain
Predicted Molecular Weight:
Observed: 21kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CSRP1
Additional Names:
CRP, CRP1, CSRP, CYRP, D1S181E, HEL-141, HEL-S-286, cysteine and glycine-rich protein 1, LIM-domain protein, cysteine-rich protein 1, epididymis luminal protein 141, epididymis secretory protein Li 286, epididymis secretory sperm binding protein
Ncbi Gene Id Number:
1465
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the cysteine-rich protein (CSRP) family. This gene family includes a group of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. The LIM/double zinc-finger motif found in this gene product occurs in proteins with critical functions in gene regulation, cell growth, and somatic differentiation. Alternatively spliced transcript variants have been described.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-771

List Size:
100 uL

List Price:

$436.00

Datasheet
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