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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Western blot analysis of CSRP2 using rabbit polyclonal CSRP2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CSRP2 gene.
CSRP2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

CSRP2 Antibody

CATALOG NUMBER: 57-459

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This CSRP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-96 amino acids from the Central region of human CSRP2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
21 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CSRP2
Additional Names:
Cysteine and glycine-rich protein 2, Cysteine-rich protein 2, CRP2, LIM domain only protein 5, LMO-5, Smooth muscle cell LIM protein, SmLIM, CSRP2, LMO5, SMLIM
Protein Accession Number:
Q16527
Protein Gi Number:
2497674
Ncbi Gene Id Number:
1466
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CSRP2 is a member of the CSRP family of genes, encoding agroup of LIM domain proteins, which may be involved in regulatoryprocesses important for development and cellular differentiation.CRP2 contains two copies of the cysteine-rich amino acid sequencemotif (LIM) with putative zinc-binding activity, and may beinvolved in regulating ordered cell growth. Other genes in thefamily include CSRP1 and CSRP3.
Background Reference 1:
Stearns, M.E., et al. Mol. Cancer Res. 1(9):631-642(2003)
Background Reference 2:
Wang, M., et al. Cancer Biol. Ther. 1(5):556-563(2002)
Background Reference 3:
Weiskirchen, R., et al. Biochem. J. 359 (PT 3), 485-496 (2001) :
Background Reference 4:
Weiskirchen, R., et al. Biochem. Biophys. Res. Commun. 274(3):655-663(2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-459

List Size:
400 ul

List Price:

$495.00

Datasheet
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