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Western blot analysis of extracts of various cell lines, using CSRP2BP antibody (22-903) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human kidney using CSRP2BP antibody (22-903) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using CSRP2BP antibody (22-903). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CSRP2BP antibody (22-903) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.

CSRP2BP Antibody

CATALOG NUMBER: 22-903

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 523-782 of human CSRP2BP (NP_065397.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HL-60
Positive Control 2:
A375
Positive Control 3:
SW480
Positive Control 4:
Mouse kidney
Positive Control 5:
Mouse heart
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 89kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CSRP2BP
Additional Names:
Cysteine-rich protein 2-binding protein, CSRP2-binding protein, ADA2A-containing complex subunit 2, ATAC2, CRP2-binding partner, CRP2BP, CSRP2BP
Ncbi Gene Id Number:
57325
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CSRP2 is a protein containing two LIM domains, which are double zinc finger motifs found in proteins of diverse function. CSRP2 and some related proteins are thought to act as protein adapters, bridging two or more proteins to form a larger protein complex. The protein encoded by this gene binds to one of the LIM domains of CSRP2 and contains an acetyltransferase domain. Although the encoded protein has been detected in the cytoplasm, it is predominantly a nuclear protein. Alternatively spliced transcript variants have been described.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-903

List Size:
100 uL

List Price:

$436.00

Datasheet
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