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Western blot analysis of extracts of HT-1080 cells, using CTAG1B antibody (23-915) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat testis using CTAG1B antibody (23-915) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using CTAG1B antibody (23-915) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of HT-1080 cells, using CTAG1B antibody (23-915) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

CTAG1B Antibody

CATALOG NUMBER: 23-915

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human CTAG1B (NP_001318.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
HT-1080
Predicted Molecular Weight:
Observed: 18kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CTAG1B
Additional Names:
cancer antigen 3, New York esophageal squamous cell carcinoma 1, NY-ESO-1, LAGE2B, LAGE-2, ESO1, CTAG1, CTAG, cancer/testis antigen 1B, CTAG1B, LAGE2, LAGE2AANDName:CTAG1BSynonyms:LAGE2B
Ncbi Gene Id Number:
1485
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is an antigen that is overexpressed in many cancers but that is also expressed in normal testis. This gene is found in a duplicated region of the X-chromosome and therefore has a neighboring gene of identical sequence.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-915

List Size:
100 uL

List Price:

$436.00

Datasheet
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