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CTAG1B Antibody

CATALOG NUMBER: 42-076

Clonality:
Polyclonal
Tested Applications:
ELISA, MA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-AELARRSLAQDAP
Conjugate:
Unconjugated
Tested Applications:
ELISA, MA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx. 28kDa band in Human Testis lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 18.0kDa according to NP_001318.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?This product has been successfully used in Microarray as described in a scientific publication (PMID: 22922091).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CTAG1B
Additional Names:
CTAG1B, cancer/testis antigen 1B, CTAG, CTAG1, ESO1, LAGE-2, LAGE2B, NY-ESO-1, New York esophageal squamous cell carcinoma 1, cancer antigen 3
Protein Accession Number:
NP_001318.1
Protein Gi Number:
4503119
Ncbi Gene Id Number:
1485
Background Reference 1:
Adams S, O1Neill DW, Nonaka D, Hardin E, Chiriboga L, Siu K, Cruz CM, Angiulli A, Angiulli F, Ritter E, Holman RM, Shapiro RL, Berman RS, Berner N, Shao Y, Manches O, Pan L, Venhaus RR, Hoffman EW, Jungbluth A, Gnjatic S, Old L, Pavlick AC, Bhardwaj N. Immunization of Malignant Melanoma Patients with Full-Length NY-ESO-1 Protein Using TLR7 Agonist Imiquimod as Vaccine Adjuvant. J Immunol. 2008 Jul 1;181(1):776-84.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-076

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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