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Western blot analysis in U251 cell line lysates (35ug/lane).
CTAG2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in U251 cell line lysates (35ug/lane).

CTAG2 Antibody

CATALOG NUMBER: 57-255

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CTAG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-149 amino acids from the Central region of human CTAG2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
21 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CTAG2
Additional Names:
Cancer/testis antigen 2, CT2, Autoimmunogenic cancer/testis antigen NY-ESO-2, Cancer/testis antigen 62, CT62, L antigen family member 1, LAGE-1, CTAG2, ESO2, LAGE1
Protein Accession Number:
O75638
Ncbi Gene Id Number:
30848
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an autoimmunogenic tumor antigen thatbelongs to the ESO/LAGE family of cancer-testis antigens. Thisprotein is expressed in a wide array of cancers including melanoma,breast cancer, bladder cancer and prostate cancer. This protein isalso expressed in normal testis tissue. An alternative open readingframe product of this gene has been described in PMID 10399963.This alternate protein, termed CAMEL, is a tumor antigen that isrecognized by melanoma-specific cytotoxic T-lymphocytes. Alternatesplicing results in multiple transcript variants. [provided byRefSeq].
Background Reference 1:
Wang, X.Y., et al. Oncol. Rep. 21(3):713-719(2009)
Background Reference 2:
Shao, Y., et al. J. Cancer Res. Clin. Oncol. 134(4):495-502(2008)
Background Reference 3:
Andrade, V.C., et al. Cancer Immun. 8,(2008) :
Background Reference 4:
Kan, T., et al. Oncology 70(1):25-33(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-255

List Size:
400 ul

List Price:

$495.00

Datasheet
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