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45-438 (5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-438 (5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

CTDSP1 Antibody

CATALOG NUMBER: 45-438

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-RGKGDQKSAASQK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 60kDa band in Human Spleen, Lung and Placenta lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 29.2kDa according to NP_067021.1 and 29.1kDa according to NP_872580.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_067021.1and NP_872580.1).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CTDSP1
Additional Names:
CTDSP1, CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1 , NLIIF, SCP1 , nuclear LIM interactor-interacting factor, small CTD phosphatase 1
Protein Accession Number:
NP_067021.1,NP_872580.1
Protein Gi Number:
10864009
Ncbi Gene Id Number:
58190
Background Reference 1:
Zhang Y, Kim Y, Genoud N, Gao J, Kelly JW, Pfaff SL, Gill GN, Dixon JE, Noel JP. Determinants for dephosphorylation of the RNA polymerase II C-terminal domain by Scp1. Mol Cell. 2006 Dec 8;24(5):759-70.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-438

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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