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Antibody is used in Western blot to detect CTDSP1-V250 in CEM tissue lysate.
Western blot analysis of CTDSP1 using CTDSP1-V250 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CTDSP1 gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Antibody is used in Western blot to detect CTDSP1-V250 in CEM tissue lysate.

CTDSP1-V250 Antibody

CATALOG NUMBER: 63-483

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This CTDSP1-V250 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-261 amino acids from the C-terminal region of human CTDSP1-V250.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CTDSP1
Additional Names:
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1, Nuclear LIM interactor-interacting factor 3, NLI-IF, NLI-interacting factor 3, Small C-terminal domain phosphatase 1, SCP1, Small CTD phosphatase 1, CTDSP1, NIF3, NLIIF, SCP1
Protein Accession Number:
Q9GZU7
Protein Gi Number:
17865510
Ncbi Gene Id Number:
58190
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CTDSP1 is a class 2C phosphatase with activity dependent on the conserved DxD motif. Expression of CTDSP1 inhibited activated transcription from several promoter-reporter gene constructs, but expression of a mutant lacking phosphatase activity enhanced transcription. Neuronal gene transcription is repressed in nonneuronal cells by the repressor element-1 (RE1)-silencing transcription factor/neuron-restrictive silencer factor (REST/NRSF; 600571) complex. REST/NRSF recruits SCPs to neuronal genes that contain RE1 elements, leading to neuronal gene silencing in nonneuronal cells. Phosphatase-inactive forms of SCP interfere with REST/NRSF function and promote neuronal differentiation of P19 stem cells. Likewise, small interfering RNA directed to the single Drosophila SCP unmasks neuronal gene expression in S2 cells. Thus, SCP activity is an evolutionarily conserved transcriptional regulator that acts globally to silence neuronal genes.
Background Reference 1:
Yeo,M., Lee,S.K., Lee,B., Ruiz,E.C., Pfaff,S.L. and Gill,G.N. Science 307 (5709): 596-600 (2005).
Background Reference 2:
Fernandes,A.O., Campagnoni,C.W., Kampf,K., Feng,J.M., Handley,V.W. J. Neurosci. Res. 75 (4): 461-471 (2004).
Background Reference 3:
Yeo,M., Lin,P.S., Dahmus,M.E. and Gill,G.N. J. Biol. Chem. 278 (28): 26078-26085 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-483

List Size:
400 ul

List Price:

$495.00

Datasheet
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