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Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
CTF1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in K562 cell line lysates (35ug/lane).

CTF1 Antibody

CATALOG NUMBER: 58-085

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CTF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-194 amino acids from the C-terminal region of human CTF1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
21 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CTF1
Additional Names:
Cardiotrophin-1, CT-1, CTF1
Protein Accession Number:
Q16619
Protein Gi Number:
2498265
Ncbi Gene Id Number:
1489
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a secreted cytokinethat induces cardiac myocyte hypertrophy in vitro. It has beenshown to bind and activate the ILST/gp130 receoptor. Two transcriptvariants encoding different isoforms have been found for this gene.
Background Reference 1:
Robador, P.A., et al. J. Hypertens. (2010) In press :
Background Reference 2:
Bailey, S.D., et al. Diabetes Care (2010) In press :
Background Reference 3:
Limongelli, G., et al. Cytokine 50(3):245-247(2010)
Background Reference 4:
Zimmerman, R.S., et al. Genet. Med. 12(5):268-278(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-085

List Size:
400 ul

List Price:

$495.00

Datasheet
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