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46-737 (0.1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-737 (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-737 (0.1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

CTLA Antibody

CATALOG NUMBER: 46-737

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: CDFNPKSSKQAKD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 35kDa and 22-23kDa bands observed in Human Brain (Frontal Cortex) lysates (calculated MW of 23.2kDa according to NP_001825.1). The observed molecular weight of ~35kDa corresponds to earlier findings in literature with different antibodies (Okamoto et al, Am J Physiol Cell Physiol. 2000 Sep;279(3):C833-51.; PMID: 10942733). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 2.5ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms of light chains a and b.
Positive Control 1:
Cat. No. 1366 - Human Frontal Lobe Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CLTA
Additional Names:
CLTA, CTLB, clathrin, light chain (Lca), LCA, clathrin, light polypeptide (Lca), clathrin, light polypeptide A, clathrin, light chain (Lcb), LCB
Protein Accession Number:
NP_001824.1,NP_009027.1,NP_001070145.1,NP_001825.1,NP_009028.1
Protein Gi Number:
4502899
Ncbi Gene Id Number:
1211;1212
Background Reference 1:
Poupon V, Girard M, Legendre-Guillemin V, Thomas S, Bourbonniere L, Philie J, Bright NA, McPherson PS. Clathrin light chains function in mannose phosphate receptor trafficking via regulation of actin assembly. Proc Natl Acad Sci USA. 2008 Jan 8;105(1):168-73. Epub 2007 Dec 28.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-737

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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