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Western blot analysis using cTnI monoclonal antibody against truncated cTnI recombinant protein.
Immunohistochemical analysis of paraffin - embedded human normal heart tissue showing cytoplasmic localization using cTnI antibody with DAB staining
Western blot analysis using cTnI monoclonal antibody against truncated cTnI recombinant protein.

cTnI Antibody [3A10A12]

CATALOG NUMBER: 32-141

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant cTnI expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:Dilution 1:500 - 1:2,000
IHC(P):Dilution 1:500 - 2,000
IHC(F):Dilution 1:500 - 2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
cTnI monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TNNI3
Additional Names:
TNNC1, Cardiac troponin ICMH7, RCM1, cTnI, CMD2A, TNNC1, CMD1FF
Protein Accession Number:
P19429
Protein Gi Number:
136213
Ncbi Gene Id Number:
7137
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
cTnI has an apparent molecular weight of 22.5 kDa. cTnI is a candidate marker with acceptable sensitivity and specificity for AMI and other cardiac diseases. Troponin, a molecule that binds to the thin filament (actin) of striated muscle fibers, acts with intracellular calcium to control the interaction of the thin filament with the thick filament (myosin), thus regulating muscle contraction. Troponin I prevents muscle contraction in the absence of calcium, which has two skeletal muscle isoforms with considerable amino acid sequence homology. cTnI contains an additional N-terminal sequence and is highly specific for myocardium.
Background Reference 1:
Cummins B and Cummins P, J Mol Cell Cardiol, 1987, 19(10):999-1010.
Background Reference 2:
Cummins B, Auckland ML, and Cummins P, Am Heart J, 1987, 113(6):1333-44.
Background Reference 3:
Darnell J, Lodish H, and Baltimore D, Molecular Cell Biology, New York, NY: Scientific American Books, 1986, 827-8.
Background Reference 4:
Larue C, Defacque-Lacquemant H, Calzolari C, et al, Mol Immunol, 1992, 29(2):271-8.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-141

List Size:
0.1 mL

List Price:

$408.00

Datasheet
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