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43-659 (0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-659 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasm/Plasma Membrane staining. The nuclear st
43-659 (0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

CTNNB1 / catenin beta-1 Antibody

CATALOG NUMBER: 43-659

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-QIPSTQFDAAHPTN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in lysates of cell line HeLa (calculated MW of 85.5kDa according to NP_001895.1). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature..Immunofluorescence: Expression of the protein seen in the cytoplasm/Plasma Membrane of U2OS cells. Recommended concentration: 10ug/ml.
Specificity:
Reported variants represent identical protein: NP_001091679.1, NP_001895.1, NP_001091680.1.
Predicted Molecular Weight:
Approx 100kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CTNNB1 / catenin beta-1
Additional Names:
CTNNB1, catenin (cadherin-associated protein), beta 1, 88kDa, CTNNB, MRD19, armadillo, catenin beta-1
Protein Accession Number:
NP_001895.1
Protein Gi Number:
113414957
Ncbi Gene Id Number:
1499
Background Reference 1:
Mazemondet O, John M, Leye S, Rolfs A, Uhrmacher AM.Elucidating the sources of β-catenin dynamics in human neural progenitor cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-659

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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