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Western blot analysis of extracts of various cell lines, using CTNND1 antibody (16-592) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using CTNND1 antibody (16-592). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CTNND1 antibody (16-592) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

CTNND1 Antibody

CATALOG NUMBER: 16-592

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 573-832 of human CTNND1 (NP_001078938.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
U-251MG
Positive Control 3:
293T
Positive Control 4:
A-549
Positive Control 5:
22Rv1
Positive Control 6:
HT-29
Predicted Molecular Weight:
Observed: 108kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CTNND1
Additional Names:
CTNND1, CAS, Catenin delta-1, CTNND, KIAA0384, p120, p120(CAS), p120(CTN), p120CAS, p120CTN, p120 catenin
Ncbi Gene Id Number:
1500
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the Armadillo protein family, which function in adhesion between cells and signal transduction. Multiple translation initiation codons and alternative splicing result in many different isoforms being translated. Not all of the full-length natures of the described transcript variants have been determined. Read-through transcription also exists between this gene and the neighboring upstream thioredoxin-related transmembrane protein 2 (TMX2) gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-592

List Size:
100 uL

List Price:

$436.00

Datasheet
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