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<strong>Figure 1 Western Blot Validation in Human and Mouse Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: CTRP3, 3565 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation with Recombinant Protein</strong><br>Loading: 30 ng of human CTRP3 recombinant protein per lane.Antibodies: CTRP3, 3565 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Mouse and Rat Tissue Lysates</strong><br>Loading: 15 μg of lysates per lane.Antibodies: CTRP3, 3565 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Western Blot Validation in Mouse Heart Tissue Lysate in the (A) absence and (B) presence of blocking peptide</strong><br>Loading: 15 μg of lysates per lane.Antibodies: CTRP3, 3565 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Immunohistochemistry Validation of CTRP3 in Mouse Heart Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CTRP3 antibody (3565) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunofluorescence Validation of CTRP3 in Mouse Heart Tissue</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling CTRP3 with 3565 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Figure 1 Western Blot Validation in Human and Mouse Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: CTRP3, 3565 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

CTRP3 Antibody

CATALOG NUMBER: 3565

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Anti-CTRP3 antibody (3565) was raised against a peptide corresponding to 16 amino acids near the center of human CTRP3.
The immunogen is located within amino acids 90-140 of CTRP3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WB: 1 μg/mL; IHC: 2 μg/mL; IF: 10 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
Multiple isoforms of CTRP3 are known to exist. CTRP3 antibody is predicted to not recognize other members of the CTRP protein family.
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Positive Control 2:
Cat. No. 1203 - A549 Cell Lysate
Positive Control 3:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 4:
Cat. No. 1282 - 3T3/NIH Cell Lysate
Predicted Molecular Weight:
Predicted: 27kD
Observed: 30 kD (Post-modyfication: 1 N-linked glycosylation)
Validation:

Recombinant Protein Test (Figure 2): Anti-CTRP3 antibodies (3565) detected human CTRP3 recombinant protein.

Isoforms:
Human CTRP3 has 3 isoforms, including isoform 1 (246aa, 27kD), isoform 2 (198aa, 22kD) and isoform 3 (319aa, 35kD). Mouse CTRP3 has one isoform (246aa, 27kD) and Rat CTRP3 also has one isoform (319aa, 35kD). 3565 can detect human, mouse and rat.
Purification:
CTRP3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CTRP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CTRP3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
C1QTNF3
Additional Names:
CTRP3 Antibody: CORS, CORCS, CTRP3, CORS26, C1ATNF3, CORS-26, UNQ753/PRO1484, Complement C1q tumor necrosis factor-related protein 3, Collagenous repeat-containing sequence 26 kDa protein
Protein Accession Number:
NP_852100
Protein Gi Number:
62739183
Ncbi Gene Id Number:
114899
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CTRP3 Antibody: Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. It has been suggested that CTRP3 may play a role in skeletal development.
Background Reference 1:
Fantuzzi. J. Allergy Clin. Immunol. 2005; 115:911-9.
Background Reference 2:
Tsao et al. Euro. J. Pharmacol. 2002; 440:213-21.
Background Reference 3:
Wong et al. Proc. Natl. Acad. Sci. USA 2004; 101:10302-7.
Background Reference 4:
Shapiro and Scherer. Curr. Biol. 1998; 8:335-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3565

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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