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Western blot analysis of CTRP4 in rat brain cell lysate with CTRP4 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Immunohistochemistry of CTRP4 in human brain tissue with CTRP4 antibody at 10 μg/mL.
Immunofluorescence of CTRP4 in human brain tissue with CTRP4 antibody at 20 μg/mL.
Western blot analysis of CTRP4 in rat brain cell lysate with CTRP4 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Trial Size

CTRP4 Antibody

CATALOG NUMBER: 3569

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
CTRP4 antibody was raised against a 16 amino acid synthetic peptide from near the center of human CTRP4.
The immunogen is located within amino acids 90 - 140 of CTRP4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
CTRP4 antibody can be used for the detection of CTRP4 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
These proteins are often highly modified post-translationally and migrate in SDS-PAGE at positions other than their predicted size.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Positive Control 2:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 3:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
CTRP4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CTRP4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CTRP4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
C1QTNF4
Additional Names:
CTRP4 Antibody: CTRP4, ZACRP4, CTRP4, Complement C1q tumor necrosis factor-related protein 4
Protein Accession Number:
AAH35628
Protein Gi Number:
23243285
Ncbi Gene Id Number:
114900
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CTRP4 Antibody: Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. Multiple isoforms of mouse CTRP4 have been reported.
Background Reference 1:
Fantuzzi G. Adipose tissue, adipokines, and inflammation. J. Allergy Clin. Immunol. 2005; 115:911-9.
Background Reference 2:
Tsao T-S, Lodish HF, and Fruebis J. ACRP30, a new hormone controlling fat and glucose metabolism. Euro. J. Pharmacol. 2002; 440:213-21.
Background Reference 3:
Wong GW, Wang J, Hug C, et al. A family of Acrp30/ adiponectin structural and functional paralogs. Proc. Natl. Acad. Sci. USA 2004; 101:10302-7.
Background Reference 4:
Shapiro L and Scherer PE. The crystal structure of a complement-1q family protein suggests an evolutionary link to tumor necrosis factor. Curr. Biol. 1998; 8:335-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3569

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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