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Immunohistochemical analysis of paraffin-embedded H. kidney section using CTSA Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis of lysates from A431, Hela, HepG2, HT-1080 cell line and human placenta, kidney tissue lysate (from left to right), using CTSA Antibody at 1:1000 at each lane.
Western blot analysis in HepG2 cell line lysates (35ug/lane).
Western blot analysis in mouse bladder tissue lysates (35ug/lane).
CTSA antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Immunohistochemical analysis of paraffin-embedded H. kidney section using CTSA Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

CTSA Antibody

CATALOG NUMBER: 55-204

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This CTSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-45 amino acids from the N-terminal region of human CTSA.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CTSA
Additional Names:
Lysosomal protective protein, Carboxypeptidase C, Carboxypeptidase L, Cathepsin A, Protective protein cathepsin A, PPCA, Protective protein for beta-galactosidase, Lysosomal protective protein 32 kDa chain, Lysosomal protective protein 20 kDa chain, CTSA, PPGB
Protein Accession Number:
P10619
Protein Gi Number:
20178316
Ncbi Gene Id Number:
5476
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CTSA encodes a glycoprotein which associates withlysosomal enzymes beta-galactosidase and neuraminidase to form acomplex of high molecular weight multimers. The formation of thiscomplex provides a protective role for stability and activity.Deficiencies in this gene are linked to multiple forms ofgalactosialidosis.
Background Reference 1:
Reich, M., et al. Immunol. Lett. 128(2):143-147(2010)
Background Reference 2:
Bonten, E.J., et al. J. Biol. Chem. 284(41):28430-28441(2009)
Background Reference 3:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Background Reference 4:
Tatano, Y., et al. J. Med. Invest. 53 (1-2), 103-112 (2006) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-204

List Size:
400 ul

List Price:

$495.00

Datasheet
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