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Western blot analysis of extracts of various cell lines, using CTSE antibody (18-763) at 1:200 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human tonsil using CTSE antibody (18-763) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using CTSE antibody (18-763) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using CTSE antibody (18-763) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using CTSE antibody (18-763).
Western blot analysis of extracts of various cell lines, using CTSE antibody (18-763) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

CTSE Antibody

CATALOG NUMBER: 18-763

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant protein of human CTSE
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Mouse spleen
Positive Control 2:
Mouse liver
Positive Control 3:
Mouse thymus
Positive Control 4:
Mouse stomach
Predicted Molecular Weight:
Observed: 43kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CTSE
Additional Names:
Cathepsin E, Cathepsin E form I, Cathepsin E form II, CTSE
Ncbi Gene Id Number:
1510
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the A1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme, an aspartic endopeptidase, may be involved in antigen processing and the maturation of secretory proteins. Elevated expression of this gene has been observed in neurodegeneration.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-763

List Size:
100 uL

List Price:

$436.00

Datasheet
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