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Western blot analysis of CTSE antibody in K562 cell line lysates (35ug/lane)
Western blot analysis of CTSE antibody in mouse stomach tissue lysates (35ug/lane)
CTSE Antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CTSE antibody in K562 cell line lysates (35ug/lane)

CTSE Antibody

CATALOG NUMBER: 62-330

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This CTSE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157-187 amino acids from the Central region of human CTSE.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CTSE
Additional Names:
Cathepsin E, Cathepsin E form I, Cathepsin E form II, CTSE
Protein Accession Number:
P14091
Protein Gi Number:
46397366
Ncbi Gene Id Number:
1510
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CTSE is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen.
Background Reference 1:
Caruso,M., Virchows Arch. 454 (3), 291-302 (2009)
Background Reference 2:
Burster,T., Biochem. Biophys. Res. Commun. 377 (4), 1299-1303 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-330

List Size:
400 ul

List Price:

$495.00

Datasheet
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