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Western blot analysis of extracts of various cell lines, using CTSG antibody (14-834) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human tonsil using CTSG antibody (14-834) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using CTSG antibody (14-834) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using CTSG antibody (14-834) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using CTSG antibody (14-834) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using CTSG antibody (14-834) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

CTSG Antibody

CATALOG NUMBER: 14-834

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 75-190 of human CTSG (NP_001902.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
Mouse kidney
Positive Control 2:
Rat kidney
Predicted Molecular Weight:
Observed: 29kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CTSG
Additional Names:
CATG, CG, cathepsin G
Ncbi Gene Id Number:
1511
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In addition, the encoded protein is antimicrobial, with bacteriocidal activity against S. aureus and N. gonorrhoeae. Transcript variants utilizing alternative polyadenylation signals exist for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-834

List Size:
100 uL

List Price:

$436.00

Datasheet
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