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Western blot analysis of CTSH Antibody in HepG2 cell line lysates (35ug/lane)
CTSH Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CTSH Antibody in HepG2 cell line lysates (35ug/lane)

CTSH Antibody

CATALOG NUMBER: 62-866

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CTSH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-54 amino acids from the N-terminal region of human CTSH.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
37 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CTSH
Additional Names:
Pro-cathepsin H, Cathepsin H mini chain, Cathepsin H, Cathepsin H heavy chain, Cathepsin H light chain, CTSH, CPSB
Protein Accession Number:
P09668
Protein Gi Number:
288558851
Ncbi Gene Id Number:
1512
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of its gene has been correlated with malignant progression of prostate tumors.
Background Reference 1:
Bunatova,K., Int. J. Biol. Markers 24 (1), 47-51 (2009)
Background Reference 2:
Decock,J., Int. J. Biol. Markers 23 (3), 161-168 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-866

List Size:
400 ul

List Price:

$495.00

Datasheet
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