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Formalin-fixed and paraffin-embedded human breast carcinoma with, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of ZR-75-1 cells using(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CTSK using rabbit polyclonal using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CTSK gene (Lane 2).
Formalin-fixed and paraffin-embedded human breast carcinoma with, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

CTSK Antibody

CATALOG NUMBER: 62-867

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Pig, Rb
Immunogen:
This CTSK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 207-237 amino acids from the Central region of human CTSK.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
37 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CTSK
Additional Names:
Cathepsin K, Cathepsin O, Cathepsin O2, Cathepsin X, CTSK, CTSO, CTSO2
Protein Accession Number:
P43235
Protein Gi Number:
1168793
Ncbi Gene Id Number:
1513
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. This gene may be subject to RNA editing.
Background Reference 1:
Lendeckel,U., Neurochem. Int. 54 (7), 410-417 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-867

List Size:
400 ul

List Price:

$495.00

Datasheet
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