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Western blot analysis in Hela cell line lysates (35ug/lane).
Western blot analysis of CTTN using rabbit polyclonal CTTN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTTN gene.
CTTN Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Hela cell line lysates (35ug/lane).

CTTN Antibody

CATALOG NUMBER: 57-227

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken
Immunogen:
This CTTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-302 amino acids from the Central region of human CTTN.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
62 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CTTN
Additional Names:
Src substrate cortactin, Amplaxin, Oncogene EMS1, CTTN, EMS1
Protein Accession Number:
Q14247
Protein Gi Number:
215273892
Ncbi Gene Id Number:
2017
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is overexpressed in breast cancer and squamouscell carcinomas of the head and neck. The encoded protein islocalized in the cytoplasm and in areas of the cell-substratumcontacts. This gene has two roles: (1) regulating the interactionsbetween components of adherens-type junctions and (2) organizingthe cytoskeleton and cell adhesion structures of epithelia andcarcinoma cells. During apoptosis, the encoded protein is degradedin a caspase-dependent manner. The aberrant regulation of this genecontributes to tumor cell invasion and metastasis. Three splicevariants that encode different isoforms have been identified forthis gene.
Background Reference 1:
Croucher, D.R., et al. Mol. Cell. Biol. 30(21):5057-5070(2010)
Background Reference 2:
Eiseler, T., et al. J. Biol. Chem. 285(24):18672-18683(2010)
Background Reference 3:
Xu, X.Z., et al. Mod. Pathol. 23(2):187-196(2010)
Background Reference 4:
Saitoh, Y., et al. Int. J. Oncol. 35(6):1277-1288(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-227

List Size:
400 ul

List Price:

$495.00

Datasheet
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