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Western blot analysis of CUEDC2 in HeLa cell lysate with CUEDC2 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of CUEDC2 in HeLa cells with CUEDC2 antibody at 5 μg/mL.
Western blot analysis of CUEDC2 in HeLa cell lysate with CUEDC2 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

CUEDC2 Antibody

CATALOG NUMBER: 4839

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
CUEDC2 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human CUEDC2.
The immunogen is located within the last 50 amino acids of CUEDC2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
CUEDC2 antibody can be used for detection of CUEDC2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificity:
CUEDC2 antibody will not cross-react with CUEDC1.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 32 kDa
Observed: 34 kDa
Purification:
CUEDC2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CUEDC2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CUEDC2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CUEDC2
Additional Names:
CUEDC2 Antibody: C10orf66, bA18I14.5, C10orf66, HOYS6, CUE domain-containing protein 2
Protein Accession Number:
NP_076945
Protein Gi Number:
148596996
Ncbi Gene Id Number:
79004
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CUEDC2 Antibody: The CUE (coupling of ubiquitin conjugation to endoplasmic reticulum degradation) domain is an evolutionarily conserved, ~40 amino acid monoubiquitin-binding domain that mediates intramolecular monoubiquitylation. CUE domains are present in eukaryotic proteins that are involved in ubiquitination and protein trafficking pathways and may be required for ubiquitination of the proteins in which they are found. CUEDC2 (CUE domain-containing protein 2) was found through a yeast two-hybrid screening as a protein that interacts with the progesterone receptor (PR) and promotes progesterone-induced PR degradation by the ubiquitin-proteasome pathway. CUEDC2 also decreases the sumoylation of PR. CUEDC2 has also been found to interact with IKK-alpha and IKK-beta and decrease the activation of NF-κB by decreasing the activation of IKK.
Background Reference 1:
Shih SC, Prag G, Francis SA, et al. A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. EMBO J. 2003; 22:1273-81.
Background Reference 2:
Ponting CP. Proteins of the endoplasmic-reticulum-associated degradation pathway: domain detection and function prediction. Biochem. J. 2000; 351:527-35.
Background Reference 3:
Prag G, Misra S, Jones EA, et al. Mechanism of ubiquitin recognition by the CUE domain of Vps9p. Cell 2003; 113:609-20.
Background Reference 4:
Kang RS, Daniels CM, Francis SA, et al. Solution structure of a CUE-ubiquitin complex reveals a conserved mode of ubiquitin binding. Cell 2003; 113:621-30.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4839

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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