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Western blot analysis in mouse heart tissue lysates (35ug/lane).
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Western blot analysis in mouse heart tissue lysates (35ug/lane).

CUL2 Antibody

CATALOG NUMBER: 58-791

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This CUL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-179 amino acids from the N-terminal region of human CUL2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
87 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CUL2
Additional Names:
Cullin-2, CUL-2, CUL2
Protein Accession Number:
Q13617
Protein Gi Number:
19863260
Ncbi Gene Id Number:
8453
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
Background Reference 1:
Park, S.W., et al. APMIS 117(12):880-885(2009)
Background Reference 2:
Argyropoulos, G., et al. Physiol. Genomics 36(2):79-88(2009)
Background Reference 3:
Barrett, J.C., et al. Nat. Genet. 40(8):955-962(2008)
Background Reference 4:
Maeda, Y., et al. J. Biol. Chem. 283(23):16084-16092(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-791

List Size:
400 ul

List Price:

$495.00

Datasheet
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