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Western blot analysis of extracts of various cell lines, using CUL4B antibody (19-640) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat liver using CUL4B antibody (19-640) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CUL4B antibody (19-640) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreas using CUL4B antibody (19-640) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using CUL4B antibody (19-640) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using CUL4B antibody (19-640). Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.
Immunofluorescence analysis of U2OS cells using CUL4B antibody (19-640).
Western blot analysis of extracts of various cell lines, using CUL4B antibody (19-640) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

CUL4B Antibody

CATALOG NUMBER: 19-640

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CUL4B (NP_003579.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Mouse liver
Positive Control 2:
Mouse kidney
Predicted Molecular Weight:
Observed: 115kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CUL4B
Additional Names:
Cullin-4B, CUL-4B, CUL4B, KIAA0695
Ncbi Gene Id Number:
8450
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-640

List Size:
100 uL

List Price:

$436.00

Out of stock

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