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Western Blot at 1:2000 dilution + human testis lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of CUL4B Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in Hela cell line lysate.
Western Blot at 1:2000 dilution + human testis lysates Lysates/proteins at 20 ug per lane.

CUL4B Antibody

CATALOG NUMBER: 60-941

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This CUL4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the Central region of human CUL4B.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
104 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CUL4B
Additional Names:
Cullin-4B, CUL-4B, CUL4B, KIAA0695
Protein Accession Number:
Q13620
Protein Gi Number:
296439468
Ncbi Gene Id Number:
8450
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the cullin family. The encodedprotein forms a complex that functions as an E3 ubiquitin ligaseand catalyzes the polyubiquitination of specific protein substratesin the cell. The protein interacts with a ring finger protein, andis required for the proteolysis of several regulators of DNAreplication including chromatin licensing and DNA replicationfactor 1 and cyclin E. Multiple transcript variants encodingdifferent isoforms have been found for this gene. [provided byRefSeq].
Background Reference 1:
Aggarwal, P., et al. Cancer Cell 18(4):329-340(2010)
Background Reference 2:
Abbas, T., et al. Mol. Cell 40(1):9-21(2010)
Background Reference 3:
Kerzendorfer, C., et al. Hum. Mol. Genet. 19(7):1324-1334(2010)
Background Reference 4:
Gascoin-Lachambre, G., et al. Placenta 31(2):151-157(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-941

List Size:
400 ul

List Price:

$495.00

Datasheet
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