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Western blot analysis of extracts of various cell lines, using CUX1 antibody (18-576) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of C6 cells using CUX1 antibody (18-576) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CUX1 antibody (18-576) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

CUX1 Antibody

CATALOG NUMBER: 18-576

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human CUX1 (NP_001189472.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:1000 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
293T
Positive Control 2:
Jurkat
Positive Control 3:
MCF7
Positive Control 4:
mouse brain
Positive Control 5:
mouse testis
Positive Control 6:
mouse kidney
Predicted Molecular Weight:
Observed: 90kDa/110kDa/250kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CUX1
Additional Names:
CUX1, CASP, CDP1, Clox, COY1, CUTL1, CCAAT displacement protein, Cut homolog, CUX, CUT, GOLIM6, Homeobox protein cux-1, p100, p200, p75, Protein CASP, CDP, Cut-like homeobox 1, Nbla10317, p110
Ncbi Gene Id Number:
1523
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants encoding different isoforms have been identified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-576

List Size:
100 uL

List Price:

$436.00

Datasheet
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