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Western blot analysis of CX3CL1 in C2C12 cell lysate with CX3CL1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Western blot analysis of CX3CL1 in C2C12 cell lysate with CX3CL1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
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CX3CL1 Antibody

CATALOG NUMBER: 2099

Clonality:
Polyclonal
Tested Applications:
ELISA, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (79%)
Immunogen:
CX3CL1 antibody was raised against a peptide corresponding to 18 amino acids near the amino terminus of human CX3CL1.
The immunogen is located within amino acids 20 - 70 of CX3CL1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP, WB
Application Note:
CX3CL1 antibody can be used for detection of CX3CL1 by Western blot at 0.5 - 2 μg/mL dilution. CX3CL1 is ofen observed migrating at 80 - 100 kDa in SDS-PAGE, presumably due to post-translational modification.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1285 - C2C12 Cell Lysate
Purification:
CX3CL1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
CX3CL1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
CX3CL1 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CX3CL1
Additional Names:
CX3CL1 Antibody: NTN, NTT, CXC3, CXC3C, SCYD1, ABCD-3, C3Xkine, fractalkine, neurotactin, FKN, A-152E5.2, Fractalkine, C-X3-C motif chemokine 1
Protein Accession Number:
AAB50014
Protein Gi Number:
1899259
Ncbi Gene Id Number:
6376
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CX3CL1 Antibody: Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues. CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively. Recent experiments have shown that CX3CL1 can suppress the production of nitrous oxide, interleukin-6, and TNF-α in activated microglia and neuronal cells, suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1.
Background Reference 1:
Bajetto A, Bonavia R, Barbero S, et al. Chemokines and their receptors in the central nervous system. Front. Neuroendocrinol. 2001; 22:147-84.
Background Reference 2:
Umehara H, Goda S, Imai T, et al. Fractalkine, a CX3C-chemokine, functions predominantly as an adhesion molecule in monocytic cell line THP-1. Immunol. Cell. Biol. 2001; 79:298-302.
Background Reference 3:
Mizuno T, Kawanokuchi J, Numata K, et al. Production and neuroprotective functions of fractalkine in the central nervous system. Brain Res. 2003; 979:65-70.
Background Reference 4:
Combadiere C, Salzwedel K, Smith ED, et al. Identification of CX3CR1. A chemotactic receptor for the human CX3C chemokine fractalkine and a fusion coreceptor for HIV-1. J. Biol. Chem.1998; 273:23799-804.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2099

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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